DNA Origami Nanopores

The combination of DNA origami nanostructures and solid-state nanopores allows unparalleled control over both the geometry and the chemical functionality of the pore. DNA nanoplates can be captured and docked onto a nanopore as shown in Fig 1 (left). The docking process is visible in the current trace (Fig 2) and the presence of the nanoplate can be determined with a current-voltage sweep. In high ionic strength conditions (1M KCl), docked nanoplates lead to a 20% drop in the current, relative to the bare nanopore. We have used this technique to study a number of different nanoplate designs and investigate their mechanical properties and ionic permeability.

DNA Origami nanopores

Figure 1. (left) 3D representation showing a cross-sectional view of a Honeycomb lattice DNA nanoplate docked onto a SiN nanopore. (right) 3D representations, AFM scans, and TEM class averages of negative stain micrographs of four different DNA origami nanoplates.

DNA Origami nanopores

Figure 2. (left) Current trace of a Honeycomb nanoplate captured onto a nanopore at 100 mV. (right) IV curve for a bare 14 nm SiN pore (blue) as well as for the same pore after a Honeycomb nanoplate was docked (red).

Related publications:
1. C. Plesa, A. Ananth, V. Linko, C. Gülcher, A. Katan, H. Dietz, C. Dekker. ACS Nano 2014, 8 (1), 35-43.